|
KMID : 0364219880310020095
|
|
Korean Journal of Zoology
1988 Volume.31 No. 2 p.95 ~ p.103
|
|
|
Alterations in the Level of Fibronectin and its Receptors during Chick Myoblast Differentiation
|
|
Chung Chang-Yong
Kang Man-Sik
|
|
|
Abstract
|
|
|
|
Alterations in the amount of fibronectin during chick myogenesis were investigated. The amount of fibronectin as measured by immunoblotting was found to decrease during the process. As a first step in answering the precise mode of change in the level of ibronedin during myogensis, the interaction of 28,000 dalton(28 kDa) amino terminal fragment of fibronectin as well as 85,000 dalton (85 kDa) fragrxient with myoblasts was examined. The specific binding of 125 l-28 kDa fragment to myoblasts was time-dependent and reached a maximum within 60 min. Unlabelled 28 kDa fragment inhibited the binding of 125 I-28 kDa fragment, whereas 85 kDa fragment containing adhesion promoting activity did not inhibit it. This finding suggests that the 28 kDa fragment interacts with the matrix assembly receptors but not with the cell adhesion receptors. Accordingly, the decrease in the level of fibronectin is likely to correlate with the fall of fibronectin receptors on the myoblasts.
|
|
|
KEYWORD
|
|
|
Myogenesis , Fibronectin receptro , Amino terminal fragment
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|